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2013学年春季学期系列学术讲座之九
题目：Engineering red fluorescent proteins for imaging of membrane potential and ion concentrations in live cells
报告人：Robert E. Campbell
Associate Professor, Department of Chemistry, University of Alberta
时间：2013年5月17日（周五），下午13:00-14:30 PM
地点：威廉希尔一楼邓祐才报告厅
Abstract:
Neuroscientists have been using voltage sensitive dyes for over the past 20 years to image changes in membrane potential associated with the propagation of action potentials. While voltage dyes have proven to be very powerful tools, they do suffer from some limitations. For example, they provide no facile means to label specific cell types and are generally applied to the whole tissue, often resulting in a relatively low signal-to-noise ratio. Accordingly, synthetic voltage dyes limit the ability of researchers to unravel the function of different types of neurons as fluorescence output cannot be attributed to a specific type of neuron. In an effort to overcome these limitations, much effort has been invested in the development of optogenetic voltage and Ca2+ neural activity reporters that enable genetic targeting to specific cell types. However, until recently, progress in this endeavor has been disappointing and has not produced voltage reporters with sufficient brightness, voltage sensitivity, and fast kinetics to be broadly useful for neuroscience research. In this seminar I will discuss some of our recent efforts to engineer an improved generation of optogenetic reporters. Our efforts have primarily focused on the creation of red fluorescent voltage and Ca2+ indicators that would enable imaging deeper into tissue with less interference from autofluorescence.
联系人：李毓龙（电话：62766915）
欢迎各位老师和同学积极参加！