生命学院学术报告

Title: Improving the precision of CRISPR/Cas9 nucleases through protein engineering

Speaker: Scot A. Wolfe (BS, CalTech; PhD, Harvard) Department of Molecular, Cell and Cancer Biology, University of Massachusetts Medical School

Time: 11:00, July 7, 2015

Venue：Room 311，New Life Sciences Building

Selected publications:

Kok et al., Reverse genetic screening reveals poor correlation between Morpholino-induced and mutant phenotypes in zebrafish. Dev Cell 2015, 32(1), 97-108.

Gupta et al., An improved predictive recognition model for Cys2-His2 zinc finger proteins. Nucleic Acids Res. 2014 42(8):4800-12.

Kearns et al., Cas9 effector-mediated regulation of transcription and differentiation in human pluripotent stem cells. Development 2014, 141(1):219-23.

Enuameh et al., Global analysis of Drosophila Cys2-His2 zinc finger proteins reveals a multitude of novel recognition motifs and binding determinants. Genome Research. 2013, 23(6), 928-40.

Gupta et al., Targeted Chromosomal Deletions and Inversions in Zebrafish. Genome Research. 2013, 23(6), 1008-17.

Zhu et al., Using Defined Finger-Finger Interfaces as Units of Assembly for Constructing Zinc Finger Nucleases. Nucleic Acids Res. 2013, 41(4), 2455-65.

Zhu et al., FlyFactorSurvey: a database of Drosophila transcription factor binding specificities determined using the bacterial one-hybrid system. Nucleic Acids Res. 2011, 39, D111-7.

Gupta et al., Zinc finger protein-dependent and -independent contributions to the in vivo off-target activity of zinc finger nucleases. Nucleic Acids Res. 2011, 39, 381-92.

Noyes et al., Analysis of homeodomain specificities allows the family-wide prediction of preferred recognition sites. Cell, 2008, 133, 1277-1289.

Meng et al., Targeted gene inactivation in zebrafish using engineered zinc finger nucleases. Nat Biotechnol, 2008, 26, 695-701.

欢迎各位老师同学积极参加！