威廉希尔学术报告
题目：Inheritance of DNA methylation at replication forks: four cytosines, two dyads, one CpG
报告人：Chenhuan Xu，Ph.D.
Postdoctoral Research Fellow,
Department of Biology, Emory University
时间：2018年6月12日（星期二）15:00-16:00
地点：金光生命科学大楼311
摘要：The faithful inheritance of the epigenome is critical for cells to maintain gene expression programs and cellular identity across cell divisions. We mapped strand-specific DNA methylation after replication forks and show maintenance of the vast majority of the DNA methylome within 20 minutes of replication and inheritance of some hemi-methylated CpG dinucleotides (hemiCpGs). Mapping the nascent DNA methylome targeted by each of the three DNA methyltransferases (DNMTs) reveals interactions between DNMTs and substrate daughter cytosines en route to maintenance methylation or hemi-methylation. Finally, we show the inheritance of hemiCpGs at short regions flanking CCCTC-binding factor (CTCF)/cohesin binding sites in pluripotent cells. Elimination of hemi-methylation causes reduced frequency of chromatin interactions emanating from these sites, suggesting a role for hemi-methylation as a stable epigenetic mark regulating CTCF-mediated chromatin interactions.
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