生命学院学术报告

Title:  The 3D Regulatory Landscape of Human Genome

报告人：Xiong Ji（季雄），Ph.D.

Post-Doctoral Research: Whitehead Institute for Biomedical Research, Massachusetts

时间：3月24日15:30-16:30

地点：新生物楼411

Abstract:

In this talk, I will describe the 3D regulatory landscape of human naive and primed embryonic stem cells. To devise this map, we identified transcriptional enhancers and insulators in these cells and placed them within the context of cohesin-associated CTCF-CTCF loops using cohesin ChIA-PET data. The CTCF-CTCF loops we identified form a chromosomal framework of insulated neighborhoods, which in turn form topologically associating domains (TADs) that are largely preserved during the transition between the naive and primed states. Regulatory changes in enhancer-promoter interactions occur within insulated neighborhoods during cell state transition. The CTCF anchor regions we identified are conserved across species, influence gene expression, and are a frequent site of mutations in cancer cells, underscoring their functional importance in cellular regulation. These 3D regulatory maps of human pluripotent cells therefore provide a foundation for future interrogation of the relationships between chromosome structure and gene control in development and disease.

欢迎各位老师同学积极参加！